PRODUCT CODE: ET1610-7

Daxx Recombinant Rabbit Monoclonal Antibody [SC54-06] (ET1610-7)

  • Recombinant

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela             <br />
Lane 2: A549 <br />
Lane 3: SW480
  • Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela             <br />
Lane 2: A549 <br />
Lane 3: SW480
  • ICC staining Daxx in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Daxx in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of Hela cells with Daxx antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Western blot analysis of Daxx on different lysates using anti-Daxx antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: A549
Lane 3: SW480

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Daxx Recombinant Rabbit Monoclonal Antibody [SC54-06] (ET1610-7)

Immunogen

Recombinant protein within human daxx aa 195-313 / 740.

Host

Rabbit

Positive Control

PANC-1, A549, Hela, SW480.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SC54-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

81 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Daxx

SYNONYMS

BING 2 antibody; BING2 antibody; CENP-C binding protein antibody; DAP 6 antibody; DAP6 antibody; DAXX antibody; DAXX_HUMAN antibody; Death associated protein 6 antibody; Death domain associated protein 6 antibody; Death domain associated protein antibody; Death domain-associated protein 6 antibody; EAP 1 antibody; EAP1 antibody; ETS1 associated protein 1 antibody; ETS1-associated protein 1 antibody; Fas binding protein antibody; Fas death domain associated protein antibody; Fas death domain-associated protein antibody; hDaxx antibody; MGC126245 antibody; MGC126246 antibody

SEQUENCE SIMILARITIES

Belongs to the DAXX family.

TISSUE SPECIFICITY

Ubiquitous.

POST-TRANSLATIONAL MODIFICATION

Sumoylated with SUMO1 on multiple lysine residues.; Phosphorylated by HIPK1 upon glucose deprivation.; Polyubiquitinated; which is promoted by CUL3 and SPOP and results in proteasomal degradation. Ubiquitinated by MDM2; inducing its degradation. Deubiquitinated by USP7; leading to stabilize it.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus, Chromosome.

FUNCTION

Activation of the cell surface receptor FAS by FAS ligand leads to the initiation of apoptosis, a process necessary for the regulation of the immune system and tissue homeostasis. FAS-mediated apoptosis appears to involve a number of divergent and overlapping pathways. Daxx appears to be a central component of a FAS-mediated apoptotic pathway involving the activation of Jun N-terminal kinase (JNK). Although Daxx itself does not contain a death domain, it specifically binds to the death domain of FAS. Overexpression of Daxx activates the JNK pathway and enhances FAS-mediated apoptosis. The Daxx apoptotic pathway acts cooperatively with but is distinct from the FAS-mediated pathway that involves interactions between the death domain-containing protein FADD and the cysteine protease FLICE. Unlike the FAS-FADD-FLICE pathway, the Daxx pathway is sensitive to the apoptotic inhibitor protein Bcl-2.