IF staining of DARS2 in whole Zebrafish tissue (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

REACTIVITY

Zebrafish

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

DARS2 Rabbit Polyclonal Antibody (ER1901-77)

Immunogen

Recombinant protein within zebrafish dars2 aa 43-321 / 660.

Host

Rabbit

Positive Control

Zebrafish tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

74 kDa

Isotype

IgG

APPLICATION DILUTION

IF:1:50-1:200

TARGET

UNIPROT #

E9QFP3

PROTEIN NAME

DARS2

SYNONYMS

Aspartyl-tRNA synthetase 2, mitochondrial

SUBCELLULAR LOCATION

Cytoplasm.

FUNCTION

The protein encoded by this gene belongs to the class-II aminoacyl-tRNA synthetase family. It is a mitochondrial enzyme that specifically aminoacylates aspartyl-tRNA. Mutations in this gene are associated with leukoencephalopathy with brainstem and spinal cord involvement and lactate elevation (LBSL).