DAAM1 Rabbit Polyclonal Antibody (ER65757)
Catalog# ER65757
Rabbit Polyclonal to DAAM1
Tested for
-
WB
Reacts with
-
Human
-
Mouse
-
Rat
Western blot analysis of DAAM1 on different lysates with Rabbit anti-DAAM1 antibody (ER65757) at 1/20,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 123 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65757) at 1/20,000 dilution was used in 5%BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HepG2 cell lysate
Lane 2: HCT 116 cell lysate
Lane 3: MDA-MB-231 cell lysate
Lane 4: PC-12 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 123 kDa
Observed band size: 110 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER65757) at 1/20,000 dilution was used in 5%BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.