PRODUCT CODE: ER1902-91

D14 Rabbit Polyclonal Antibody (ER1902-91)

Applications

  • WB

  • IHC-P

  • IF

REACTIVITY

  • Rice

Western blot analysis of D14 on rice tissue  lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of D14 on rice tissue  lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunofluorescence staining of paraffin- embedded rice tissue using anti-D14 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH 6.0) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody () at 1/100 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded  rice  tissue using anti-D14 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody () at 1/100 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
Western blot analysis of D14 on rice tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-91, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • IF

REACTIVITY

  • Rice

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

D14 Rabbit Polyclonal Antibody (ER1902-91)

Immunogen

Synthetic peptide within n-terminal rice d14.

Host

Rabbit

Positive Control

Rice.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

Predicted band size 33 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1000

  • IHC-P

  • 1:50-1:200

  • IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

D14

SYNONYMS

Strigolactone esterase D1 antibody; Protein DWARF 14 antibody,; rotein DWARF 88, antibody; Protein HIGH-TILLERING DWARF 2 antibody ; D14 antibody

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Involved in strigolactone (SL) signaling pathway. May function downstream of SL synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of SL to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Strigolactone-dependent association of D14 with D3 and D53 (a repressor of SL signaling) triggers D53 ubiquitination and degradation . Hydrolyzes the butenolide ring of SLs . A reaction product D-OH is trapped in the cavity of D14, inducing the interaction with SLR1, and probably with other proteins such as D3 and D53 . Contributes to the negative regulation of gibberellin signaling .