Cytokeratin 7 Mouse Monoclonal Antibody [3-G3-D8] (EM0702)
Catalog# EM0702
Mouse Monoclonal to Cytokeratin 7
Tested for
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WB
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IHC-P
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IF-Cell
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IF-Tissue
Reacts with
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Human
Knock-Out or Knock-Down validated
☑ Knockout (KO)
All lanes: Western blot analysis of Cytokeratin-7 with anti-Cytokeratin-7 antibody [3-G3-D8] (EM0702) at 1:1,000 dilution.
Lane 1/2: Wild-type Hela whole cell lysate.
Lane 3/4: Cytokeratin-7 fragment 1 knockout Hela whole cell lysate.
Lane 5/6: Cytokeratin-7 fragment 2 knockout Hela whole cell lysate.
EM0702 was shown to specifically react with Cytokeratin-7 in wild-type Hela cells. No band was observed when Cytokeratin-7 knockout samples were tested. Wild-type and Cytokeratin-7 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM0702, 1/1,000) and Loading control antibody(Rabbit anti-Vinculin, ET1705-94, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.
All lanes: Western blot analysis of Cytokeratin-7 with anti-Cytokeratin-7 antibody [3-G3-D8] (EM0702) at 1:1,000 dilution.
Lane 1/2: Wild-type Hela whole cell lysate.
Lane 3/4: Cytokeratin-7 fragment 1 knockout Hela whole cell lysate.
Lane 5/6: Cytokeratin-7 fragment 2 knockout Hela whole cell lysate.
EM0702 was shown to specifically react with Cytokeratin-7 in wild-type Hela cells. No band was observed when Cytokeratin-7 knockout samples were tested. Wild-type and Cytokeratin-7 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (EM0702, 1/1,000) and Loading control antibody(Rabbit anti-Vinculin, ET1705-94, 1/5,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG-HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 1 hour at room temperature.