Cytokeratin 6 Recombinant Rabbit Monoclonal Antibody [SN71-07] (ET1611-70)
Catalog# ET1611-70
Rabbit Monoclonal to Cytokeratin 6
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Human
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Rat
Lane 1: HaCaT cell lysate
Lane 2: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-70) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cytokeratin 6 Recombinant Rabbit Monoclonal Antibody [SN71-07] (ET1611-70)
Immunogen
Recombinant protein within human cytokeratin 6 aa 1-118 / 564.
Host
Rabbit
Positive Control
HaCaT cell lysate, A431 cell lysate, A431, rat skin tissue, human tonsil tissue, human breast cancer tissue, human lung squamous cell carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN71-07
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IF-Cell
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1:100-1:500
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IF-Tissue
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1:100-1:500
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IHC-P
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1:50-1:200
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FC
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 60 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cytokeratin 6
SYNONYMS
CK6 antibody;CK 6A antibody;CK 6B antibody;CK 6C antibody;CK 6D antibody;CK 6E antibody;CK-6B antibody;CK-6C antibody;CK-6E antibody;Cytokeratin 6a antibody;Cytokeratin 6B antibody;Cytokeratin 6C antibody;Cytokeratin 6D antibody;Cytokeratin 6E antibody;Cytokeratin-6B antibody;Cytokeratin-6C antibody;Cytokeratin-6E antibody;K2C6C_HUMAN antibody;K6a keratin antibody;K6b keratin antibody;K6C antibody;K6c keratin antibody;K6d keratin antibody;K6e keratin antibody;Keratin antibody;Keratin K6h antibody;Keratin type II cytoskeletal 6A antibody;Keratin type II cytoskeletal 6B antibody;Keratin type II cytoskeletal 6C antibody;Keratin type II cytoskeletal 6D antibody;Keratin type II cytoskeletal 6E antibody;Keratin-6C antibody;KRT6A antibody;KRT6B antibody;KRT6C antibody;KRT6D antibody;KRT6E antibody;type II cytoskeletal 6C antibody;Type-II keratin Kb12 antibody
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
TISSUE SPECIFICITY
Expressed in the corneal epithelium (at protein level).
SUBCELLULAR LOCATION
Keratin filament, cytosol, extracellular exosome, nucleus, membrane.
FUNCTION
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins, which are useful markers of tissue differentiation, also aid in the characterization of malignant tumors. IL-1 and TNFα induce transcription of cytokeratin 6 in epidermal keratinocytes via the C/EBP β transcription factor. In humans, multiple isoforms of cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies.
CITATIONS
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Wu, J. J., Zhu, S., Gu, F., Valencak, T. G., Liu, J. X., & Sun, H. Z.
Cross-tissue single-cell transcriptomic landscape reveals the key cell subtypes and their potential roles in the nutrient absorption and metabolism in dairy cattle
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IF
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Dairy cattle
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Citation
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Ye, H., Li, X., Lin, J., Yang, P., & Su, M.
CD98hc has a pivotal role in maintaining the immuno-barrier integrity of basal layer cells in esophageal epithelium
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IHC
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Human
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Citation
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Xue, M. Y., Wu, J. J., Xie, Y. Y., Zhu, S. L., Zhong, Y. F., Liu, J. X., & Sun, H. Z.
Investigation of fiber utilization in the rumen of dairy cows based on metagenome-assembled genomes and single-cell RNA sequencing
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IF
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Dairy cows
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Citation
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