Cytokeratin 5+6 Recombinant Rabbit Monoclonal Antibody [PSH0-68] (HA721456)
Catalog# HA721456
Rabbit Monoclonal to Cytokeratin 5+6
Tested for
-
WB
-
IF-Cell
-
FC
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Cytokeratin 5+6 on different lysates with Rabbit anti-Cytokeratin 5+6 antibody (HA721456) at 1/1,000 dilution.
Lane 1: A431 cell lysate
Lane 2: NCI-H226 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 55 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721456) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: A431 cell lysate
Lane 2: NCI-H226 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 60 kDa
Observed band size: 55 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721456) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.