Cytokeratin 5+6 Mouse Monoclonal Antibody [A2A12] (EM1901-07)
Catalog# EM1901-07
Mouse Monoclonal to Cytokeratin 5+6
Tested for
-
WB
-
IHC-P
-
FC
Reacts with
-
Human
-
Rat
Western blot analysis of Cytokeratin 5+6 on different lysates with Mouse anti-Cytokeratin 5+6 antibody (EM1901-07) at 1/1,000 dilution.
Lane 1: HT-29 cell lysate
Lane 2: A549 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60/62 kDa
Observed band size: 50 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-07) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HT-29 cell lysate
Lane 2: A549 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 60/62 kDa
Observed band size: 50 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1901-07) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.