Applications
-
WB
-
ICC/IF
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cytokeratin 16 Recombinant Rabbit Monoclonal Antibody [SC52-09] (ET1610-17)
Immunogen
Synthetic peptide within huamn cytokeratin 16 aa 27-75 / 473.
Host
Rabbit
Positive Control
Human skin tissue lysates, Hela, HepG2, A431, human tonsil tissue, human lung carcinoma tissue, human breast carcinoma tissue, human esophagus tissue, human breast tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC52-09
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
51 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Cytokeratin 16
SYNONYMS
CK 16 antibody;CK-16 antibody;CK16 antibody;Cytokeratin-16 antibody;Cytokeratin16 antibody;FNEPPK antibody;Focal non epidermolytic palmoplantar keratoderma antibody;K 16 antibody;K16 antibody;K1C16_HUMAN antibody;K1CP antibody;Keratin 1 type I antibody;Keratin 16 antibody;Keratin antibody;Keratin type I cytoskeletal 16 antibody;Keratin-16 antibody;Keratin16 antibody;KRT 16 antibody;Krt16 antibody;KRT16A antibody;NEPPK antibody;PC1 antibody;type I cytoskeletal 16 antibody
SEQUENCE SIMILARITIES
Belongs to the intermediate filament family.
TISSUE SPECIFICITY
Expressed in the corneal epithelium (at protein level).
SUBCELLULAR LOCATION
Cytoskeleton, intermediate filament, cytosol, extracellular exosome, nucleus.
FUNCTION
Cytokeratins comprise a diverse group of intermediate filament proteins that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. The cytokeratin proteins play a critical role in differentiation, as well as tissue specialization and function, to maintain the overall structural integrity of epithelial cells. Cytokeratins are also useful markers in identifying the origin of metastatic tumors. Cytokeratin 16 is expressed in benign stratified squamous epithelium and squamous cell carcinoma of the head and neck, as well as luminal cells of mammary gland and sweat ducts. It is absent in noninvasive breast carcinomas and normal breast tissue. Mutations in the Cytokeratin 16 gene cause various diseases, including pachyonychia congenita type 1 (PC1), nonepidermolytic palmoplantar keratoderma (NEPPK) and unilateral palmoplantar verrucous nevus (UPVN).