Western blot analysis of Cytokeratin 14 on A431 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM1901-33, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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Belongs to the intermediate filament family.
Expressed in the corneal epithelium (at protein level). Detected in the basal layer, lowered within the more apically located layers specifically in the stratum spinosum, stratum granulosum but is not detected in stratum corneum. Strongly expressed in the outer root sheath of anagen follicles but not in the germinative matrix, inner root sheath or hair. Found in keratinocytes surrounding the club hair during telogen.
A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.; Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.
Keratin 14 is a member of the type I keratin family of intermediate filament proteins. Keratin 14 is also known as cytokeratin-14 (CK-14) or keratin-14 (KRT14). In humans it is encoded by the KRT14 gene. Keratin 14 is usually found as a heterodimer with type II keratin 5 and form the cytoskeleton of epithelial cells. Cytokeratin 14 is found in squamous epithelial basal cells, myoepithelium, some glandular epithelia, and mesothelial cells. Anti-Cytokeratin 14 is useful for distinguishing squamous cell carcinomas from other epithelial tumors, and for classifying metaplastic breast carcinomas. The Cytokeratin 14 (IHC555) antibody is intended for qualified laboratories to qualitatively identify by light microscopy the presence of associated antigens in sections of formalin-fixed, paraffin-embedded tissue sections using IHC test methods. Use of this antibody is indicated, subsequent to clinical differential diagnoses of diseases, as an aid in the identification of squamous cell carcinomas within the context of antibody panels, the patient’s clinical history and other diagnostic tests evaluated by a qualified pathologist.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.