Cytochrome C Rabbit Polyclonal Antibody (R1510-41)
Catalog# R1510-41
Rabbit Polyclonal to Cytochrome C
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: L-929 cell lysate (20 µg/Lane)
Lane 3: C6 cell lysate (20 µg/Lane)
Lane 4: Human liver tissue lysate (40 µg/Lane)
Lane 5: Mouse liver tissue lysate (40 µg/Lane)
Lane 6: Rat liver tissue lysate (40 µg/Lane)
Predicted band size: 12 kDa
Observed band size: 12 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (R1510-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Cytochrome C Rabbit Polyclonal Antibody (R1510-41)
Immunogen
Synthetic peptide within human cytochrome c aa 1-50 / 105.
Host
Rabbit
Positive Control
HeLa cell lysate, L-929 cell lysate, C6 cell lysate, human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human spleen tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse spleen tissue, mouse heart tissue, mouse kidney tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 12kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cytochrome C
SYNONYMS
CYC antibody;CYC_HUMAN antibody;CYCS antibody;Cytochrome c antibody;Cytochrome c somatic antibody;HCS antibody;THC4 antibody
SEQUENCE SIMILARITIES
Belongs to the cytochrome c family.
POST-TRANSLATIONAL MODIFICATION
Binds 1 heme group per subunit.; Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.
SUBCELLULAR LOCATION
Mitochondrion intermembrane space
FUNCTION
Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
CITATIONS
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Zhang, Y., Li, B. X., Mao, Q. Z., Zhuo, J. C., Huang, H. J., Lu, J. B., Zhang, C. X., Li, J. M., Chen, J. P., & Lu, G.
The JAK-STAT pathway promotes persistent viral infection by activating apoptosis in insect vectors
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WB
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Laodelphax striatellus
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Citation
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Muhammad A
iTRAQ-based quantitative proteomics analysis of molecular mechanisms associated with Bombyx mori (Lepidoptera) larval midgut response to BmNPV in susceptible and near-isogenic strains
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WB
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Bombyx mori
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Citation
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Butyrate inhibits the mitochondrial complex Ι to mediate mitochondria-dependent apoptosis of cervical cancer cells
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IF
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Human
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Citation
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