PRODUCT CODE: R1510-41

Cytochrome C Rabbit Polyclonal Antibody (R1510-41)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis on mouse brain(1) mouse heart(2) and mouse kidney(3) cell lysates using anti-Cytochrome C rabbit polyclonal antibody.
  • Western blot analysis on mouse brain(1) mouse heart(2) and mouse kidney(3) cell lysates using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunocytochemical staining of Hela cells using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunocytochemical staining of HepG2 cells using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunocytochemical staining of MCF-7 cells using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunocytochemical staining of 293 cells using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human spleen tissue using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse liver tissue using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse spleen tissue using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-Cytochrome C rabbit polyclonal antibody.
  • Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-Cytochrome C rabbit polyclonal antibody.
  • Flow cytometric analysis of Hela cells with Cytochrome C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis on mouse brain(1) mouse heart(2) and mouse kidney(3) cell lysates using anti-Cytochrome C rabbit polyclonal antibody.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Cytochrome C Rabbit Polyclonal Antibody (R1510-41)

Immunogen

Peptide

Host

Rabbit

Positive Control

Hela, HepG2, 293, MCF-7, human spleen tissue, human kidney tissue, human liver tissue, mouse liver tissue, mouse spleen tissue, mouse heart tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

12kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Cytochrome C

SYNONYMS

CYC antibody; CYC_HUMAN antibody; CYCS antibody; Cytochrome c antibody; Cytochrome c somatic antibody; HCS antibody; THC4 antibody

SEQUENCE SIMILARITIES

Belongs to the cytochrome c family.

POST-TRANSLATIONAL MODIFICATION

Binds 1 heme group per subunit.; Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

SUBCELLULAR LOCATION

Mitochondrion intermembrane space

FUNCTION

Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.

CITATIONS

  • Yu, Haizhong et al.

    iTRAQ-based quantitative proteomics analysis of molecular mechanisms associated with Bombyx mori (Lepidoptera) larval midgut response to BmNPV in susceptible and near-isogenic strains. | Journal of Proteomics [2017]