Cystatin F Recombinant Rabbit Monoclonal Antibody [PSH09-00] (HA723053)
Catalog# HA723053
Rabbit Monoclonal to Cystatin F
Tested for
-
WB
-
IHC-Fr
Reacts with
-
Human
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Cystatin F on different lysates with Rabbit anti-Cystatin F antibody (HA723053) at 1/2,000 dilution.
Lane 1: U-937 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (negative) (20 µg/Lane)
Predicted band size: 16 kDa
Observed band size: 20 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723053) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Cystatin F on different lysates with Rabbit anti-Cystatin F antibody (HA723053) at 1/2,000 dilution.
Lane 1: U-937 cell lysate (20 µg/Lane)
Lane 2: K-562 cell lysate (negative) (20 µg/Lane)
Predicted band size: 16 kDa
Observed band size: 20 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723053) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.