Cyclin D1 Recombinant Rabbit Monoclonal Antibody [PD01-64] (HA721322)
Catalog# HA721322
Rabbit Monoclonal to Cyclin D1
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Rat
Western blot analysis of Cyclin D1 on different lysates with Rabbit anti-Cyclin D1 antibody (HA721322) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: K-562 cell lysate (negative)
Lane 3: A431 cell lysate
Lane 4: Neuro-2a cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lane 7: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 35 kDa
Exposure time: 20 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721322) at 1/5,000 dilution and competitor's antibody at 1/5,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cyclin D1 Recombinant Rabbit Monoclonal Antibody [PD01-64] (HA721322)
Immunogen
Recombinant protein within human cyclin d1 aa 200-295 (c terminal).
Host
Rabbit
Positive Control
MCF7 cell lysate, A431 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, SH-SY5Y cell lysate, Neuro-2a, MCF7, human colon carcinoma tissue, human testis tissue, NIH/3T3.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PD01-64
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:200
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IF-Cell
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1:2,000
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FC
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1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 34 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cyclin D1
SYNONYMS
AI327039 antibody;B cell CLL/lymphoma 1 antibody;B cell leukemia 1 antibody;B cell lymphoma 1 protein antibody;B-cell lymphoma 1 protein antibody;BCL 1 antibody;BCL-1 antibody;BCL-1 oncogene antibody;BCL1 antibody;BCL1 oncogene antibody;ccnd1 antibody;cyclind1 antibody;CCND1/FSTL3 fusion gene antibody;CCND1/FSTL3 fusion gene, included antibody;CCND1/IGHG1 fusion gene antibody;CCND1/IGHG1 fusion gene, included antibody;CCND1/IGLC1 fusion gene antibody;CCND1/IGLC1 fusion gene, included antibody;CCND1/PTH fusion gene antibody;CCND1/PTH fusion gene, included antibody;CCND1_HUMAN antibody;cD1 antibody;Cyl 1 antibody;D11S287E antibody;G1/S specific cyclin D1 antibody;G1/S-specific cyclin-D1 antibody;Parathyroid adenomatosis 1 antibody;PRAD1 antibody;PRAD1 oncogene antibody;U21B31 antibody
SEQUENCE SIMILARITIES
Belongs to the cyclin family. Cyclin D subfamily.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex.; Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation (By similarity). Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Membrane, Mitochondrion
FUNCTION
The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDKs (Cyclin-dependent kinase). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Micrograph of cyclin D1 staining in a mantle cell lymphoma. Immunohistochemical staining of cyclin D1 antibodies is used to diagnose mantle cell lymphoma. Cyclin D1 has been found to be overexpressed in breast carcinoma. Its potential use as a biomarker was suggested.
CITATIONS
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Qian XL, Meng D, Liu H, et al
Proliferation Inhibitory Activity of Quinones from Blaps rynchopetera Defense Secretion on Colorectal Tumor Cells
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WB
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Human
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Citation
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The overexpression of actin related protein 2/3 complex subunit 1B(ARPC1B) promotes the ovarian cancer progression via activation of the Wnt/β-catenin signaling pathway
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WB
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Human
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Citation
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