Cyclin A2 Mouse Monoclonal Antibody [N0-E3] (M1511-5)
Catalog# M1511-5
Mouse Monoclonal to Cyclin A2
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
Mouse
-
Rat
Positive control:
Lane 1: 293
Lane 2: F9
Lane 3: PC12
Lane 4: Hela
Specifications
Product Type
Mouse monoclonal primary
Product Name
Cyclin A2 Mouse Monoclonal Antibody [N0-E3] (M1511-5)
Immunogen
Recombinant protein within human p53 aa 50-432.
Host
Mouse
Positive Control
293 cell lysates, F9 cell lysates, PC12 cell lysates, Hela cell lysates, human tonsil tissue, human spleen tissue, human uterus tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
N0-E3
RRID
APPLICATION DILUTION
-
WB
-
1:500
-
IF-Cell
-
1:200
-
IHC-P
-
1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 48 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
Cyclin A2
SYNONYMS
CCN1 antibody;CCNA antibody;Ccna2 antibody;CCNA2_HUMAN antibody;Cyclin A2 antibody;Cyclin-A antibody;Cyclin-A2 antibody
SEQUENCE SIMILARITIES
Belongs to the cyclin family. Cyclin AB subfamily.
DEVELOPMENTAL STAGE
Accumulates steadily during G2 and is abruptly destroyed at mitosis. Not detected during the G1 phase of the cell cycle. It accumulates during the DNA synthesis/S phase and disappears as cells progress into mitosis, between prophase and metaphase (at protein level).
POST-TRANSLATIONAL MODIFICATION
Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.
SUBCELLULAR LOCATION
Nucleus, Cytoplasm.
FUNCTION
The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. The best-characterized cyclin complex is the mitotic cyclin B/Cdc2 p34 kinase, the active component of maturing promoting factor. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin- dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F and retinoblastoma protein, p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34.
