PRODUCT CODE: ET1612-8

Cyclin A1+ Cyclin A2 Recombinant Rabbit Monoclonal Antibody [SD2053] (ET1612-8)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

Western blot analysis of Cyclin A1+ Cyclin A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: HepG2 cell lysate
  • Western blot analysis of Cyclin A1+ Cyclin A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: HepG2 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-Cyclin A1+ Cyclin A2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1612-8, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Cyclin A1+ Cyclin A2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1612-8, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Cyclin A1+ Cyclin A2 Recombinant Rabbit Monoclonal Antibody [SD2053] (ET1612-8)

Immunogen

Recombinant protein within human cyclin a1 + cyclin a2 aa 253-440 / 465 a1同源性6959%.

Host

Rabbit

Positive Control

Hela, HepG2, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD2053

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52/49 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Cyclin A1+ Cyclin A2

SYNONYMS

CCN1 antibody; CCNA antibody; CCNA1 antibody; CCNA2 antibody; CT146 antibody; Cyclin-A antibody; Cyclin-A1 antibody; Cyclin-A2 antibody

SEQUENCE SIMILARITIES

Belongs to the cyclin family. Cyclin AB subfamily.

DEVELOPMENTAL STAGE

Accumulates steadily during G2 and is abruptly destroyed at mitosis. Not detected during the G1 phase of the cell cycle. It accumulates during the DNA synthesis/S phase and disappears as cells progress into mitosis, between prophase and metaphase (at protein level).

POST-TRANSLATIONAL MODIFICATION

Polyubiquitinated via 'Lys-11'-linked ubiquitin by the anaphase-promoting complex (APC/C), leading to its degradation by the proteasome. Deubiquitinated and stabilized by USP37 enables entry into S phase.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

The critical role that the family of regulatory proteins known as cyclins play in eukaryotic cell cycle regulation is well established. Cyclin A accumulates prior to cyclin B in the cell cycle, appears to be involved in control of S phase and has been shown to associate with cyclin-dependent kinase-2 (Cdk2). In addition, cyclin A has been implicated in cell transformation and is found in complexes with E1A, transcription factors DRTF1 and E2F, and retinoblastoma protein p110. A second form of cyclin A, named cyclin A1 because of its high sequence homology to Xenopus cyclin A1, is most highly expressed in germ cells. It has been proposed that cyclin A1 can associate with Cdk2, p39 and Cdc2 p34. Cyclin A2 is a member of the highly conserved cyclin family. Cyclins regulate CDK kinases and different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin A2 is expressed in all tissues tested, in contrast to cyclin A1, which is present only in germ cells. The Cyclin A2 protein binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 also functions in the transition to DNA replication and synthesis phases of the cell cycle and is quickly destroyed as the cell moves into mitosis.

CITATIONS

  • Fan, W. J., Ding, H., Ch......

    Fan, W. J., Ding, H., Chen, X. X., & Yang, L. (2021). All-Trans Retinoic Acid Potentiates Antitumor Efficacy of Cisplatin by Increasing Differentiation of Cancer Stem-Like Cells in Cervical Cancer. Annals of clinical and laboratory science, 51(1), 22–29.