CXCR4 Recombinant Rabbit Monoclonal Antibody [JE03-35] (HA722304)
Catalog# HA722304
Rabbit Monoclonal to CXCR4
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WB
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IF-Cell
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IHC-P
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Human
Lane 1: Jurkat cell lysate (hot lysis)
Lane 2: HeLa cell lysate (hot lysis)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 45 kDa
Exposure time: 25 second; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722304) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CXCR4 Recombinant Rabbit Monoclonal Antibody [JE03-35] (HA722304)
Immunogen
Recombinant protein within human cxcr4 aa 253-352 / 352.
Host
Rabbit
Positive Control
Jurkat cell lysate, HeLa cell lysate, HeLa, human tonsil tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE03-35
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 40 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CXCR4
SYNONYMS
C-X-C chemokine receptor type 4 antibody;CD184 antibody;CD184 antigen antibody;Chemokine (C X C motif) receptor 4 antibody;Chemokine CXC Motif Receptor 4 antibody;CXC-R4 antibody;CXCR-4 antibody;CXCR4 antibody;CXCR4_HUMAN antibody;D2S201E antibody;FB22 antibody;Fusin antibody;HM89 antibody;HSY3RR antibody;LAP 3 antibody;LAP3 antibody;LCR1 antibody;LESTR antibody;Leukocyte derived seven transmembrane domain receptor antibody;Leukocyte-derived seven transmembrane domain receptor antibody;Lipopolysaccharide associated protein 3 antibody;Neuropeptide Y receptor Y3 antibody;NPY3R antibody;NPYR antibody;NPYRL antibody;NPYY3 antibody;NPYY3R antibody;Probable G protein coupled receptor lcr1 homolog antibody;SDF 1 receptor antibody;SDF-1 receptor antibody;SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR antibody;Stromal cell derived factor 1 receptor antibody;Stromal cell-derived factor 1 receptor antibody;WHIM antibody;WHIMS antibody
SUBCELLULAR LOCATION
Cell membrane, Cell junction, Early endosome, Late endosome, Lysosome.
FUNCTION
C-X-C chemokine receptor type 4 (CXCR-4) also known as fusin or CD184 (cluster of differentiation 184) is a protein that in humans is encoded by the CXCR4 gene. The protein is a CXC chemokine receptor. CXCR-4 is an alpha-chemokine receptor specific for stromal-derived-factor-1 (SDF-1 also called CXCL12), a molecule endowed with potent chemotactic activity for lymphocytes. CXCR4 is one of several chemokine co-receptors that HIV can use to infect CD4+ T cells. HIV isolates that use CXCR4 are traditionally known as T-cell tropic isolates. Typically, these viruses are found late in infection. It is unclear as to whether the emergence of CXCR4-using HIV is a consequence or a cause of immunodeficiency.
CITATIONS
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Da-Yong Hou,et al
In vivo self-assembled bispecific fluorescence probe for early detection of bladder cancer and metastasis
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IHC
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Mouse
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Citation
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