PRODUCT CODE: ET1704-97

CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33] (ET1704-97)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

Western blot analysis of CXCR3 on Hela cell using anti-CXCR3 antibody at 1/1,000 dilution.
  • Western blot analysis of CXCR3 on Hela cell using anti-CXCR3 antibody at 1/1,000 dilution.
  • ICC staining CXCR3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CXCR3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CXCR3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of CXCR3 on Hela cell using anti-CXCR3 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CXCR3 Recombinant Rabbit Monoclonal Antibody [JA61-33] (ET1704-97)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, A431, HepG2, HUVEC.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA61-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

41 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CXCR3

SYNONYMS

C-X-C chemokine receptor type 3 antibody; CD 183 antibody; CD182 antibody; CD183 antibody; Chemokine (C X C motif) receptor 3 antibody; Chemokine (C X C) receptor 3 antibody; Chemokine CXC Motif Receptor 3 antibody; CKR L2 antibody; CKR-L2 antibody; CKRL2 antibody; CMKAR3 antibody; CXC-R3 antibody; CXCR-3 antibody; CXCR3 antibody; CXCR3_HUMAN antibody; G Protein Coupled Receptor 9 antibody; G protein-coupled receptor 9 antibody; GPR9 antibody; Interferon-inducible protein 10 receptor antibody; IP-10 receptor antibody; IP10 antibody; IP10 R antibody; IP10 receptor antibody; IP10-R antibody; IP10R antibody; Mig R antibody; Mig receptor antibody; Mig-R antibody; MIGR antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family.

TISSUE SPECIFICITY

Isoform 1 and isoform 2 are mainly expressed in heart, kidney, liver and skeletal muscle. Isoform 1 is also expressed in placenta. Isoform 2 is expressed in endothelial cells. Expressed in T-cells (at protein level).

POST-TRANSLATIONAL MODIFICATION

Sulfation on Tyr-27 and Tyr-29 is essential for CXCL10 binding and subsequent signal transduction induction.; N-glycosylated.

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

The C-X-C or α chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell derived factor-1 (SDF-1). SDF-1 was originally described as a pre-B cell stimulatory factor, but has since been shown to function as a potent chemoattractant for T cells and monocytes but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven pass transmembrane domain proteins which include IL-8RA, IL-8RB, CXCR-3 and fusin (also designated LESTR or CXCR-4). CXCR-3, also known as IP-10/MIG receptor, mediates Ca2+ mobilization and chemotaxis in response to the C-X-C chemokines IP-10 and MIG. CXCR-3 is highly expressed in IL-2-activated T lymphocytes, but not in resting T lymphocytes, B lymphocytes, monocytes or granulocytes.