Lane 1: SH-SY5Y
Lane 2: 293T
Lane 3: Mouse testis tissue
Rabbit polyclonal primary
Cullin-3 Rabbit Polyclonal Antibody (ER1706-70)
Synthetic peptide within human cullin-3 aa 724-768.
SH-SY5Y, 293T, mouse testis tissue lysate, human kidney tissue, human placenta tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified
CUL 3 antibody; Cul-3 antibody; cul3 antibody; CUL3_HUMAN antibody; Cullin-3 antibody; Cullin3 antibody; KIAA0617 antibody; PHA2E antibody
Belongs to the cullin family.
Brain, spermatozoa, and testis (at protein level). Widely expressed.
Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.
Golgi apparatus. Nucleus.
Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.