PRODUCT CODE: ER1706-70

Cullin-3 Rabbit Polyclonal Antibody (ER1706-70)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: SH-SY5Y  <br />
Lane 2: 293T<br />
Lane 3: Mouse testis tissue
  • Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution.<br />
Positive control:<br />
Lane 1: SH-SY5Y  <br />
Lane 2: 293T<br />
Lane 3: Mouse testis tissue
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Cullin-3 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with Cullin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: 293T
Lane 3: Mouse testis tissue

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Cullin-3 Rabbit Polyclonal Antibody (ER1706-70)

Immunogen

Synthetic peptide within human cullin-3 aa 724-768.

Host

Rabbit

Positive Control

SH-SY5Y, 293T, mouse testis tissue lysate, human kidney tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

89 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Cullin-3

SYNONYMS

CUL 3 antibody; Cul-3 antibody; cul3 antibody; CUL3_HUMAN antibody; Cullin-3 antibody; Cullin3 antibody; KIAA0617 antibody; PHA2E antibody

SEQUENCE SIMILARITIES

Belongs to the cullin family.

TISSUE SPECIFICITY

Brain, spermatozoa, and testis (at protein level). Widely expressed.

POST-TRANSLATIONAL MODIFICATION

Neddylated. Attachment of NEDD8 is required for the E3 ubiquitin-protein ligase activity of the BCR complex. Deneddylated via its interaction with the COP9 signalosome (CSN) complex.

SUBCELLULAR LOCATION

Golgi apparatus. Nucleus.

FUNCTION

Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.