PRODUCT CODE: EM1701-36

CTHRC1 Mouse Monoclonal Antibody [5H1] (EM1701-36)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of CTHRC1 on CTHRC1 recombinant protein using anti-CTHRC1 antibody at 1/500 dilution.
  • Western blot analysis of CTHRC1 on CTHRC1 recombinant protein using anti-CTHRC1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CTHRC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CTHRC1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-CTHRC1 antibody. Counter stained with hematoxylin.
Western blot analysis of CTHRC1 on CTHRC1 recombinant protein using anti-CTHRC1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CTHRC1 Mouse Monoclonal Antibody [5H1] (EM1701-36)

Immunogen

Recombinant protein within human cthrc1 aa 1-243 / 243.

Host

Mouse

Positive Control

CTHRC1 recombinant protein, rat large intestine tissue, human kidney tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

5H1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CTHRC1

SYNONYMS

Collagen triple helix repeat containing 1 antibody; Collagen triple helix repeat containing protein 1 antibody; Collagen triple helix repeat-containing protein 1 antibody; CTHR1_HUMAN antibody; Cthrc1 antibody; NMTC1 protein antibody; Protein NMTC1 antibody

TISSUE SPECIFICITY

Isoform 1 is expressed in calcified atherosclerotic plaque and chondrocyte-like cells.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated.

SUBCELLULAR LOCATION

Extracellular matrix. Secreted.

FUNCTION

CTHRC1 (collagen triple helix repeat containing 1) is a 243 amino acid secreted protein that localizes to the extracellular matrix and contains one collagen-like domain. Expressed as two alternatively spliced isoforms, the first of which is expressed in calcified atherosclerotic plaque and chondrocyte-like cells, CTHRC1 is thought to function as a negative regulator of collagen matrix deposition and may also play a role in the stabilization of ligand-receptor interactions in the Wnt pathway. CTHRC1 is widely present in a variety of cancers, including melanoma and breast carcinoma, and may participate in cancer cell migration and cancer tissue invasion and metastasis. The gene encoding CTHRC1 maps to human chromosome 8, which consists of nearly 146 million base pairs, houses more than 800 genes and is associated with a variety of diseases and malignancies.

CITATIONS

  • Yang, Xiao-Mei et al.

    CTHRC1 promotes human colorectal cancer cell proliferation and invasiveness by activating Wnt/PCP signaling. | International Journal of Clinical and Experimental Pathology [2015]

  • Ma, Ming-Ze et al.

    CTHRC1 acts as a prognostic factor and promotes invasiveness of gastrointestinal stromal tumors by activating Wnt/PCP-Rho signaling. | Neoplasia (New York, N.Y.) [2014]

  • Li, Jun et al.

    Autocrine CTHRC1 activates hepatic stellate cells and promotes liver fibrosis by activating TGF-�_ signaling. | EbioMedicine [2019]