PRODUCT CODE: ER1802-69

CTGF Rabbit Polyclonal Antibody (ER1802-69)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CTGF on mouse smooth muscle tissue lysate using anti-CTGF antibody at 1/500 dilution.
  • Western blot analysis of CTGF on mouse smooth muscle tissue lysate using anti-CTGF antibody at 1/500 dilution.
  • ICC staining CTGF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CTGF in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining CTGF in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CTGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer tissue using anti-CTGF antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CTGF antibody. Counter stained with hematoxylin.
Western blot analysis of CTGF on mouse smooth muscle tissue lysate using anti-CTGF antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CTGF Rabbit Polyclonal Antibody (ER1802-69)

Immunogen

Recombinant protein within human ctgf aa 147-293 / 349.

Host

Rabbit

Positive Control

Mouse smooth muscle tissue lysate, HepG2, HUVEC, SiHa, human appendix tissue, human thyroid gland cancer tissue, human uterus tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CTGF

SYNONYMS

CCN 2 antibody; CCN family member 2 antibody; CCN2 antibody; Connective tissue growth factor antibody; Ctgf antibody; CTGF_HUMAN antibody; Hcs 24 antibody; Hcs24 antibody; Hypertrophic chondrocyte specific protein 24 antibody; Hypertrophic chondrocyte-specific gene product 24 antibody; Hypertrophic chondrocyte-specific protein 24 antibody IBP-8 antibody; IGF-binding protein 8 antibody; IGFBP-8 antibody; IGFBP8 antibody; Insulin like Growth Factor Binding Protein 8 antibody; Insulin-like growth factor-binding protein 8 antibody; MGC102839 antibody; NOV 2 antibody; NOV2 antibody

SEQUENCE SIMILARITIES

Belongs to the CCN family.

TISSUE SPECIFICITY

Expressed in bone marrow and thymic cells. Also expressed one of two Wilms tumors tested.

SUBCELLULAR LOCATION

Extracellular region or secreted.

FUNCTION

Connective tissue growth factor (CTGF, also known as hypertrophic chondrocyte-specific gene product 24 or Hcs24), is a member of the CCN family of immediate early proteins, which are involved in cell proliferation, migration and matrix production. CTGF is a cysteine-rich peptide that is secreted by endothelial cells, fibroblasts, smooth muscle cells and myofibroblasts. Its expression is increased in various human and animal fibrotic diseases. Specifically, CTGF has been observed to be strongly upregulated in human proliferative and fibrogenic renal disease. In addition, CTGF is a growth factor for vascular smooth muscle cells (VSMC) and may play a similar role in promoting VSMC growth and migration in vitro.

CITATIONS

  • Wu, Ming et al.

    Reduced asymmetric dimethylarginine accumulation through inhibition of the type I protein arginine methyltransferases promotes renal fibrosis in obstructed kidneys. | Faseb Journal : Official Publication of the Federation of American Societies for Experimental Biology [2019]