CSF-1-R Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH06-70] (HA751099)
Catalog# HA751099
Rabbit Monoclonal to CSF-1-R
Tested for
-
WB
-
IF-Cell
Reacts with
-
Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of CSF-1-R on different lysates with Rabbit anti-CSF-1-R antibody (HA751099) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: J774A.1 cell lysate
Lane 3: Neuro-2a cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 109 kDa
Observed band size: 90-160 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751099) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CSF-1-R on different lysates with Rabbit anti-CSF-1-R antibody (HA751099) at 1/2,000 dilution.
Lane 1: RAW264.7 cell lysate
Lane 2: J774A.1 cell lysate
Lane 3: Neuro-2a cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 109 kDa
Observed band size: 90-160 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751099) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.