CRMP1 Recombinant Rabbit Monoclonal Antibody [JU73-30] (ET7106-86)
Catalog# ET7106-86
Rabbit Monoclonal to CRMP1
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WB
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IF-Cell
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
Lane 1: Human brain tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 62 kDa
Observed band size: 62 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-86) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CRMP1 Recombinant Rabbit Monoclonal Antibody [JU73-30] (ET7106-86)
Immunogen
Synthetic peptide within human crmp1 aa 489-538 / 572.
Host
Rabbit
Positive Control
Human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, N2A, SHG-44, SH-SY5Y, rat brain tissue, mouse brain tissue, mouse cerebellum tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU73-30
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 62 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CRMP1
SYNONYMS
Collapsin response mediator protein 1 antibody;CRMP 1 antibody;CRMP-1 antibody;Crmp1 antibody;Dihydropyrimidinase like 1 antibody;Dihydropyrimidinase related protein 1 antibody;Dihydropyrimidinase-related protein 1 antibody;DPYL1_HUMAN antibody;DPYSL1 antibody;DRP 1 antibody;DRP-1 antibody;DRP1 antibody;ULIP-3 antibody;Ulip3 antibody;Unc-33-like phosphoprotein 3 antibody
SEQUENCE SIMILARITIES
Belongs to the metallo-dependent hydrolases superfamily. Hydantoinase/dihydropyrimidinase family.
TISSUE SPECIFICITY
Brain.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Ser-522 enhances CRMP1-mediated alteration of FLNA ternary structure and FLNA dissociation from F-actin.
SUBCELLULAR LOCATION
Cytoplasm. Cytoskeleton.
FUNCTION
Collapsin response mediator proteins (CRMPs), including CRMP-1 (DRP-1), CRMP-2 (DRP-2 or TOAD64), CRMP-3 (DRP-4), CRMP-4 (DRP-3) and CRMP-5 (DRP-5), mediate signal transduction after exposure of neural cells to the axon guidance molecule Semaphorin 3A (SEMA3A)/collapsin. CRMPs are present in the developing cerebral cortex and neocortical neurons and are responsive to SEMA3A. In the adult brain, the expression of CRMPs is dramatically downregulated. However, they remain expressed in structures that retain their capacity for differentiation and plasticity. CRMP-1 is also involved in cancer invasion and metastasis.
CITATIONS
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Mitochondrial protein prohibitin promotes learning memory recovery in mice following intracerebral hemorrhage via CAMKII/CRMP signaling pathway
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WB
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Mouse
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Citation
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