CrkL Rabbit Polyclonal Antibody (HA500536)
Catalog# HA500536
Rabbit Polyclonal to CrkL
Tested for
-
WB
-
IF-Cell
-
FC
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
☑ Relative expression (RE)
Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (HA500536) at 1/2,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Lane 6: A431 cell lysate (20 µg/Lane)
Lane 7: HL-60 cell lysate (low expression) (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: Mouse thymus tissue lysate (40 µg/Lane)
Lane 10: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 37 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500536) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of CrkL on different lysates with Rabbit anti-CrkL antibody (HA500536) at 1/2,000 dilution.
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: 293T cell lysate (20 µg/Lane)
Lane 3: Jurkat cell lysate (20 µg/Lane)
Lane 4: HT-29 cell lysate (20 µg/Lane)
Lane 5: THP-1 cell lysate (20 µg/Lane)
Lane 6: A431 cell lysate (20 µg/Lane)
Lane 7: HL-60 cell lysate (low expression) (20 µg/Lane)
Lane 8: C6 cell lysate (20 µg/Lane)
Lane 9: Mouse thymus tissue lysate (40 µg/Lane)
Lane 10: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 34 kDa
Observed band size: 37 kDa
Exposure time: 40 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500536) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.