CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] (ET1703-85)
Catalog# ET1703-85
Rabbit Monoclonal to CRISPR-Cas9 SP
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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Streptococcus pyogenes
Western blot analysis of CRISPR-Cas9 SP on 293T transfected with Cas9 cell lysates with Rabbit anti-CRISPR-Cas9 SP antibody (ET1703-85) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 158 kDa
Observed band size: 158 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1703-85) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] (ET1703-85)
Immunogen
Recombinant protein within streptococcus pyogenes cas9 aa 730-990 / 1368.
Host
Rabbit
Positive Control
293T transfected with Cas9 cell lysates.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM11-55
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:50-1:200
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
158 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CRISPR-Cas9 SP
SYNONYMS
Cas9 antibody;CRISPR-associated endonuclease Cas9/Csn1 antibody;CRISPR-Cas9/Csn1 antibody;csn1 antibody;SpyCas9 antibody
SEQUENCE SIMILARITIES
Belongs to the CRISPR-associated protein Cas9 family. Subtype II-A subfamily.
FUNCTION
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.
CITATIONS
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An optimized prime editing system for efficient modification of the pig genome
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WB
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Pig
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Citation
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Doxycycline-dependent Cas9-expressing pig resources for conditional in vivo gene nullification and activation
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IHC
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IF-tissue
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pig
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Citation
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Li Zhifang,et al
Engineering a transposon-associated TnpB-ωRNA system for efficient gene editing and phenotypic correction of a tyrosinaemia mouse model
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WB
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Mouse
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Citation
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Jian Wang,et al
A platform of functional studies of ESCC-associated gene mutations identifies the roles of TGFBR2 in ESCC progression and metastasis
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Mouse
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Citation
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Phosphorylation of ATF2 promotes odontoblastic differentiation via intrinsic HAT activity
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Ge Weikai,et al
In vivo evaluation of guide-free Cas9-induced safety risks in a pig model
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FC
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Pig
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Citation
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