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PRODUCT CODE: ET1703-85

CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] (ET1703-85)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Streptococcus pyogenes

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Western blot analysis of CRISPR-Cas9 SP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: 293 cell lysates transfected with CRISPR-Cas9<br /> Lane 2: 293T cell lysates transfected with CRISPR-Cas9
  • Western blot analysis of CRISPR-Cas9 SP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: 293 cell lysates transfected with CRISPR-Cas9<br /> Lane 2: 293T cell lysates transfected with CRISPR-Cas9
Western blot analysis of CRISPR-Cas9 SP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-85, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysates transfected with CRISPR-Cas9
Lane 2: 293T cell lysates transfected with CRISPR-Cas9

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Streptococcus pyogenes

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] (ET1703-85)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293 cell lysate transfected with CRISPR-Cas9, 293T cell lysate transfected with CRISPR-Cas9.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-55

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

158 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q99ZW2

PROTEIN NAME

CRISPR-Cas9 SP

SYNONYMS

Cas9 antibody; CRISPR-associated endonuclease Cas9/Csn1 antibody; CRISPR-Cas9/Csn1 antibody; csn1 antibody; SpyCas9 antibody

SEQUENCE SIMILARITIES

Belongs to the CRISPR-associated protein Cas9 family. Subtype II-A subfamily.

FUNCTION

CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.