Positive control:
Lane 1: 293 cell lysates transfected with CRISPR-Cas9
Lane 2: 293T cell lysates transfected with CRISPR-Cas9
Applications
-
WB
-
ICC
-
IF
-
IHC-P
-
FC
REACTIVITY
-
Streptococcus pyogenes
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CRISPR-Cas9 SP Recombinant Rabbit Monoclonal Antibody [JM11-55] (ET1703-85)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
293 cell lysate transfected with CRISPR-Cas9, 293T cell lysate transfected with CRISPR-Cas9.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM11-55
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
158 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
CRISPR-Cas9 SP
SYNONYMS
Cas9 antibody; CRISPR-associated endonuclease Cas9/Csn1 antibody; CRISPR-Cas9/Csn1 antibody; csn1 antibody; SpyCas9 antibody
SEQUENCE SIMILARITIES
Belongs to the CRISPR-associated protein Cas9 family. Subtype II-A subfamily.
FUNCTION
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.