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CRISPR-Cas9 Rabbit Polyclonal Antibody (R1504-1)

Price: Regular price $315.00

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Catalog# R1504-1

Rabbit Polyclonal to CRISPR-Cas9

Tested for

  • WB

  • ELISA

  • IF-Cell

Reacts with

  • Staphylococcus aureus

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Specifications

Product Type

Rabbit polyclonal primary

Product Name

CRISPR-Cas9 Rabbit Polyclonal Antibody (R1504-1)

Immunogen

Recombinant protein within crispr-cas9 aa 54-141.

Host

Rabbit

Positive Control

CRISPR-Cas9 transfected 293 cells.

Conjugation

Unconjugated

Clonality

Polyclonal

RRID

AB_3073315

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • ELISA

  • 1:10,000

  • IF-Cell

  • 1:200-1:500

PROPERTIES

Form

Liquid

Storage Condition

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 158 kDa

Isotype

IgG

TARGET

UNIPROT #

Q99ZW2

PROTEIN NAME

CRISPR-Cas9

SYNONYMS

Cas9 antibody;CRISPR-associated endonuclease Cas9/Csn1 antibody;CRISPR-Cas9/Csn1 antibody;csn1 antibody;SpyCas9 antibody

FUNCTION

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.

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