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PRODUCT CODE: R1504-1

CRISPR-Cas9 Rabbit Polyclonal Antibody (R1504-1)

Applications

  • WB

  • ELISA

  • ICC

REACTIVITY

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Western blot analysis on CRISPR-Cas9 transfected 293 cells (+) and non transtected 293 cells (-).
  • Western blot analysis on CRISPR-Cas9 transfected 293 cells (+) and non transtected 293 cells (-).
  • Immunofluorescence analysis of CRISPR-Cas9 transfected 293 cells. The cell expressing CRISPR-Cas9 can be detected using rabbit anti- CRISPR-Cas9 (1:500) followed by FITC (1:1000) goat anti-rabbit IgG.
Western blot analysis on CRISPR-Cas9 transfected 293 cells (+) and non transtected 293 cells (-).

Applications

  • WB

  • ELISA

  • ICC

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

CRISPR-Cas9 Rabbit Polyclonal Antibody (R1504-1)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

CRISPR-Cas9 transfected 293 cells

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • ELISA

  • 1:10,000

  • ICC

  • 1:200-1:500

TARGET

UNIPROT #

Q99ZW2

PROTEIN NAME

CRISPR-Cas9

SYNONYMS

Cas9 antibody; CRISPR-associated endonuclease Cas9/Csn1 antibody; CRISPR-Cas9/Csn1 antibody; csn1 antibody; SpyCas9 antibody

FUNCTION

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.