PRODUCT CODE: EM50804

CRISPR-Cas9 Mouse Monoclonal Antibody [8-A6-6] (EM50804)

Applications

  • WB

  • ICC

REACTIVITY

Western blot analysis of CRISPR-Cas9 on 293T cell lysates using anti-CRISPR-Cas9 antibody at 1/5000 dilution. Lane 1: CRISPR-Cas9 transfected 293 cells lysate  Lane 2: Non-transfected 293 cells lysate
  • Western blot analysis of CRISPR-Cas9 on 293T cell lysates using anti-CRISPR-Cas9 antibody at 1/5000 dilution. Lane 1: CRISPR-Cas9 transfected 293 cells lysate  Lane 2: Non-transfected 293 cells lysate
  • Immunofluorescence staining CRISPR-Cas9 in CRISPR-Cas9 transfected 293T cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of CRISPR-Cas9 on 293T cell lysates using anti-CRISPR-Cas9 antibody at 1/5000 dilution. Lane 1: CRISPR-Cas9 transfected 293 cells lysate Lane 2: Non-transfected 293 cells lysate

Applications

  • WB

  • ICC

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

CRISPR-Cas9 Mouse Monoclonal Antibody [8-A6-6] (EM50804)

Immunogen

Recombinant protein within streptococcus pyogenes crispr-cas9 aa1-200.

Host

Mouse

Positive Control

Human 293T cells transfected with CRISPR-Cas9 lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

8-A6-6

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

Isotype

IgM

APPLICATION DILUTION

  • WB

  • 1:5,000

  • ICC

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

CRISPR-Cas9

SYNONYMS

Cas9 antibody; CRISPR-associated endonuclease Cas9/Csn1 antibody; CRISPR-Cas9/Csn1 antibody; csn1 antibody; SpyCas9 antibody

FUNCTION

The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.