PRODUCT CODE: ET7107-69

CPS1 Recombinant Rabbit Monoclonal Antibody [JB40-33] (ET7107-69)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of CPS1 on SiHa cell using anti-CPS1 antibody at 1/500 dilution.
  • Western blot analysis of CPS1 on SiHa cell using anti-CPS1 antibody at 1/500 dilution.
  • ICC staining CPS1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-CPS1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-CPS1 antibody. Counter stained with hematoxylin.
Western blot analysis of CPS1 on SiHa cell using anti-CPS1 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CPS1 Recombinant Rabbit Monoclonal Antibody [JB40-33] (ET7107-69)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HepG2, SiHa, human liver tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB40-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

165 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CPS1

SYNONYMS

Carbamoyl phosphate synthase [ammonia] antibody; Carbamoyl phosphate synthase [ammonia] mitochondrial antibody; Carbamoyl phosphate synthase antibody; Carbamoyl phosphate synthetase 1 antibody; Carbamoyl phosphate synthetase 1 mitochondrial antibody; Carbamoyl phosphate synthetase I antibody; Carbamoyl-phosphate synthase [ammonia] antibody; Carbamoyl-phosphate synthetase I antibody; Carbamoylphosphate synthase antibody; Carbamoylphosphate synthetase 1 antibody; Carbamoylphosphate synthetase I antibody; CPS 1 antibody; Cps1 antibody; CPSase 1 antibody; CPSase I antibody; CPSASE1 antibody; CPSM_HUMAN antibody; mitochondrial antibody; MS738 antibody

TISSUE SPECIFICITY

Primarily in the liver and small intestine.

POST-TRANSLATIONAL MODIFICATION

Undergoes proteolytic cleavage in the C-terminal region corresponding to the loss of approximately 12 AA residues from the C-terminus.; Succinylated at Lys-287 and Lys-1291. Desuccinylated at Lys-1291 by SIRT5, leading to activation (By similarity).; Glutarylated. Glutarylation levels increase during fasting. Deglutarylated by SIRT5 at Lys-55, Lys-219, Lys-412, Lys-889, Lys-892, Lys-915, Lys-1360 and Lys-1486, leading to activation.

SUBCELLULAR LOCATION

Mitochondrion.

FUNCTION

The multicomplex protein, carbamoyl-phosphate synthetase-aspartate carbamoyl transferase-dihydro-orotase (CAD), consists of three distinct proteins, carbamoyl phosphate synthetase 2 (CPS2), aspartate transcarbamylase, and dihydro-orotase, which catalyze the second and third steps of pyrimidine biosynthesis. CAD is allosterically regulated by the phosphorylation of CPS2 by cyclic AMP-dependent protein kinase, and this activation enables CPS2 to catalyze the rate-limiting step of pyrimidine synthesis. CAD is expressed in brain and skeletal muscle. A related protein, carbamoyl phosphate synthetase 1 (CPS1) is expressed in liver. CPS1 catalyzes the rate-limiting step in the urea cycle, and deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia.