CPS1 Recombinant Rabbit Monoclonal Antibody [JB40-33] (ET7107-69)
Catalog# ET7107-69
Rabbit Monoclonal to CPS1
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WB
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IF-Cell
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: Mouse small intestine tissue lysate (20 µg/Lane)
Lane 4: Rat liver tissue lysate (20 µg/Lane)
Predicted band size: 165 kDa
Observed band size: 150 kDa
Exposure time: Lane 1-2: 25 seconds; Lane 3-4: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-69) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CPS1 Recombinant Rabbit Monoclonal Antibody [JB40-33] (ET7107-69)
Immunogen
Recombinant protein within human cps1 aa 82-204 / 1,500.
Host
Rabbit
Positive Control
HepG2 cell lysate, HeLa cell lysate, mouse small intestine tissue lysate, rat liver tissue lysate, HepG2, human liver tissue, mouse liver tissue, rat liver tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JB40-33
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 165 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CPS1
SYNONYMS
Carbamoyl phosphate synthase [ammonia] antibody;Carbamoyl phosphate synthase [ammonia] mitochondrial antibody;Carbamoyl phosphate synthase antibody;Carbamoyl phosphate synthetase 1 antibody;Carbamoyl phosphate synthetase 1 mitochondrial antibody;Carbamoyl phosphate synthetase I antibody;Carbamoyl-phosphate synthase [ammonia] antibody;Carbamoyl-phosphate synthetase I antibody;Carbamoylphosphate synthase antibody;Carbamoylphosphate synthetase 1 antibody;Carbamoylphosphate synthetase I antibody;CPS 1 antibody;Cps1 antibody;CPSase 1 antibody;CPSase I antibody;CPSASE1 antibody;CPSM_HUMAN antibody;mitochondrial antibody;MS738 antibody
TISSUE SPECIFICITY
Primarily in the liver and small intestine.
POST-TRANSLATIONAL MODIFICATION
Undergoes proteolytic cleavage in the C-terminal region corresponding to the loss of approximately 12 AA residues from the C-terminus.; Succinylated at Lys-287 and Lys-1291. Desuccinylated at Lys-1291 by SIRT5, leading to activation (By similarity).; Glutarylated. Glutarylation levels increase during fasting. Deglutarylated by SIRT5 at Lys-55, Lys-219, Lys-412, Lys-889, Lys-892, Lys-915, Lys-1360 and Lys-1486, leading to activation.
SUBCELLULAR LOCATION
Mitochondrion, Nucleus.
FUNCTION
The multicomplex protein, carbamoyl-phosphate synthetase-aspartate carbamoyl transferase-dihydro-orotase (CAD), consists of three distinct proteins, carbamoyl phosphate synthetase 2 (CPS2), aspartate transcarbamylase, and dihydro-orotase, which catalyze the second and third steps of pyrimidine biosynthesis. CAD is allosterically regulated by the phosphorylation of CPS2 by cyclic AMP-dependent protein kinase, and this activation enables CPS2 to catalyze the rate-limiting step of pyrimidine synthesis. CAD is expressed in brain and skeletal muscle. A related protein, carbamoyl phosphate synthetase 1 (CPS1) is expressed in liver. CPS1 catalyzes the rate-limiting step in the urea cycle, and deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia.
CITATIONS
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He, J., Lin, M., Zhang, X., Zhang, R., Tian, T., Zhou, Y., Dong, W., Yang, Y., Sun, X., Dai, Y., Xu, Y., Zhang, Z., Xu, M., Lei, Q. Y., Xu, Y., & Lv, L.
TET2 is required to suppress mTORC1 signaling through urea cycle with therapeutic potential
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IHC
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WB
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Mouse
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Citation
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