COX2 Mouse Monoclonal Antibody [A3F8] (EM1902-11)
Catalog# EM1902-11
Mouse Monoclonal to COX2
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WB
-
IHC-P
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FC
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Human
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Rat
Specifications
Product Type
Mouse monoclonal primary
Product Name
COX2 Mouse Monoclonal Antibody [A3F8] (EM1902-11)
Immunogen
Synthetic peptide within c-terminal human cox2.
Host
Mouse
Positive Control
A549 cell lysates, rat spinal cord tissue lysates,rat bladder tissue, human lung carcinoma tissue, human womb tissue, A549.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A3F8
RRID
APPLICATION DILUTION
-
WB:1:500-1:1,000
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IHC-P:1:50-1:200
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FC:1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein G affinity purified.
Molecular Weight
Predicted band size: 69 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
COX2
SYNONYMS
COX 2 antibody;COX-2 antibody;COX2 antibody;Cyclooxygenase 2 antibody;Cyclooxygenase 2b antibody;Cyclooxygenase antibody;Cyclooxygenase-2 antibody;Cyclooxygenase2 antibody;EC 1.14.99.1 antibody;fj02a10 antibody;Glucocorticoid-regulated inflammatory cyclooxygenase antibody;Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase antibody;GRIPGHS antibody;hCox 2 antibody;Macrophage activation-associated marker protein P71/73 antibody;OTTHUMP00000033524 antibody;PES-2 antibody;PGG/HS antibody;PGH synthase 2 antibody;PGH2_HUMAN antibody;PGHS 2 antibody;PGHS-2 antibody;PGHS2 antibody;PHS 2 antibody;PHS II antibody;PHS2 antibody;Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody;Prostaglandin endoperoxide synthase 2 antibody;Prostaglandin G/H synthase 2 antibody;Prostaglandin G/H synthase 2 precursor antibody;Prostaglandin G/H synthase and cyclooxygenase antibody;Prostaglandin G/H synthase antibody;Prostaglandin H2 synthase 2 antibody;prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) antibody;Prostaglandin-endoperoxide synthase 2 antibody;PTGS2 antibody;ptgs2a antibody;TIS10 antibody;TIS10 protein antibody;unp1239 antibody;wu:fj02a10 antibody
SEQUENCE SIMILARITIES
Belongs to the prostaglandin G/H synthase family.
POST-TRANSLATIONAL MODIFICATION
S-nitrosylation by NOS2 (iNOS) activates enzyme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-526.; Acetylated at Ser-565 by SPHK1. During neuroinflammation, acetylation by SPHK1 promotes neuronal secretion of specialized preresolving mediators (SPMs), especially 15-R-lipoxin A4, which results in an increase of phagocytic microglia.
SUBCELLULAR LOCATION
Microsome membrane, endoplasmic reticulum membrane.
FUNCTION
Cytochrome c oxidase subunit 2, also known as cytochrome c oxidase polypeptide II, is a protein that in humans is encoded by the MT-CO2 gene.Cytochrome c oxidase subunit II, abbreviated COXII, COX2, COII, or MT-CO2, is the second subunit of cytochrome c oxidase. It is also one of the three mitochondrial DNA (mtDNA) encoded subunits (MT-CO1, MT-CO2, MT-CO3) of respiratory complex IV. The MT-CO2 gene is located on the p arm of mitochondrial DNA at position 12 and it spans 683 base pairs.[5] The MT-CO2 gene produces a 25.6 kDa protein composed of 227 amino acids.MT-CO2 is a subunit of the enzyme Cytochrome c oxidase (EC 1.9.3.1) (Complex IV), an oligomeric enzymatic complex of the mitochondrial respiratory chain involved in the transfer of electrons from cytochrome c to oxygen. In eukaryotes this enzyme complex is located in the mitochondrial inner membrane; in aerobic prokaryotes it is found in the plasma membrane. The enzyme complex consists of 3-4 subunits (prokaryotes) to up to 13 polypeptides (mammals). The N-terminal domain of cytochrome C oxidase contains two transmembrane alpha-helices. The structure of MT-CO2 is known to contain one redox center and a binuclear copper A center (CuA). The CuA is located in a conserved cysteine loop at 196 and 200 amino acid positions and conserved histidine at 204. Several bacterial MT-CO2 have a C-terminal extension that contains a covalently bound haem c.
