CoREST Recombinant Rabbit Monoclonal Antibody [JE35-15] (HA721585)
Catalog# HA721585
Rabbit Monoclonal to CoREST
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of CoREST on different lysates with Rabbit anti-CoREST antibody (HA721585) at 1/1,000 dilution.
Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: U-2 OS cell lysate
Lane 8: HT-29 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65/26 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721585) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: K-562 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: MCF7 cell lysate
Lane 4: HeLa cell lysate
Lane 5: HepG2 cell lysate
Lane 6: A549 cell lysate
Lane 7: U-2 OS cell lysate
Lane 8: HT-29 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 65 kDa
Observed band size: 65/26 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721585) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.