Applications
-
WB
-
IHC-P
-
ICC
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
Connexin-37 Rabbit Polyclonal Antibody (ER1901-85)
Immunogen
Synthetic peptide corresponding to n terminal human connexin-37.
Host
Rabbit
Positive Control
293T cell lysates, HepG2, LOVO, SKOV-3, rat brain tissue, Human small intestine tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
37 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500
-
IHC-P
-
1:500-1:200
-
ICC
-
1:500-1:200
-
FC
-
1:500-1:100
TARGET
UNIPROT #
PROTEIN NAME
Connexin-37
SYNONYMS
Connexin 37 antibody; Connexin-37 antibody; Cx37 antibody; CXA4_HUMAN antibody; Gap junction alpha 4 protein antibody; Gap junction alpha-4 protein antibody; gap junction protein, alpha 4 37kDa (connexin 37) antibody; gap junction protein, alpha 4, 37kD (connexin 37) antibody; Gap junction protein, alpha 4, 37kDa antibody; Gja4 antibody; OTTHUMP00000004181 antibody; OTTHUMP00000004182 antibody
SEQUENCE SIMILARITIES
Belongs to the connexin family. Alpha-type (group II) subfamily.
TISSUE SPECIFICITY
Expressed in multiple organs and tissues, including heart, uterus, ovary, and blood vessel endothelium.
SUBCELLULAR LOCATION
Cell junction, Cell membrane, Gap junction, Membrane.
FUNCTION
Connexins (Cxs) are a family of transmembrane proteins comprising 21 members in humans that forms gap junction channels (GJCs). Connexins are divided in four groups: α, β, γ and δ. Cx37 is also named α4 since it is the fourth connexin of the alpha group that was identified. Connexins do not function as single proteins; six connexins assemble into connexons (hemi-channels), and two connexons from neighboring cells dock in the extracellular space to form a full gap junction channel. Connexins are responsible for mediating direct communication between adjacent cells by the exchange of ions and small signaling molecules and cytosolic factors1,3. Their structure consists of four transmembrane helices, two extracellular loops, a cytoplasmic loop, a cytoplasmic NH2- and COOH-termini. Connexin-37 is encoded by the GJA4 gene and is expressed in vascular endothelial cells. The protein is responsible for regulating and controlling the growth, proliferation and regeneration of the vascular endothelium by mediating the signal transduction between smooth muscle and endothelial cells. Cx37 knockout mice have a tendency for developing endarterium atheromatous plaques4. Also, a polymorphism in the gene that encodes for Cx37, is associated with increased risk for atherosclerosis and myocardial infarction1.