PRODUCT CODE: ER1803-58

Connexin-32 Rabbit Polyclonal Antibody (ER1803-58)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of Connexin-32 on different lysates using anti-Connexin-32 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
              Lane 2: A431<br />
 Lane 3: Human skin tissue
  • Western blot analysis of Connexin-32 on different lysates using anti-Connexin-32 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
              Lane 2: A431<br />
 Lane 3: Human skin tissue
  • Flow cytometric analysis of SKOV-3 cells with Connexin-32 antibody at 1/100 dilution (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Connexin-32 on different lysates using anti-Connexin-32 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3M
Lane 2: A431
Lane 3: Human skin tissue

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Connexin-32 Rabbit Polyclonal Antibody (ER1803-58)

Immunogen

Synthetic peptide within mouse connexin-32 aa 230-265.

Host

Rabbit

Positive Control

PC-3M, A431, human skin tissue, SKOV-3.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

32 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Connexin-32

SYNONYMS

Charcot Marie Tooth neuropathy X linked antibody; CMTX 1 antibody; CMTX antibody; CMTX1 antibody; Connexin-32 antibody; Connexin32 antibody; CX 32 antibody; Cx32 antibody; CXB1_HUMAN antibody; GAP junction 28 kDa liver protein antibody; Gap junction beta 1 protein antibody; Gap junction beta-1 protein antibody; Gap junction protein beta 1 32kD antibody; Gap junction protein beta 1 antibody; GJB 1 antibody; GJB1 antibody

SEQUENCE SIMILARITIES

Belongs to the connexin family. Beta-type (group I) subfamily.

SUBCELLULAR LOCATION

Plasma membrane.

FUNCTION

This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.