PRODUCT CODE: ET1609-68

COL1A1/Collagen Ⅰ Recombinant Rabbit Monoclonal Antibody [ST58-04] (ET1609-68)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Cow

Western blot analysis of COL1A1/Collagen Ⅰ on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of COL1A1/Collagen Ⅰ on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-COL1A1/Collagen Ⅰ antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-COL1A1/Collagen Ⅰ antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1609-68, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of COL1A1/Collagen Ⅰ on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1609-68, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Cow

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

COL1A1/Collagen Ⅰ Recombinant Rabbit Monoclonal Antibody [ST58-04] (ET1609-68)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta tissue lysates, human kidney tissue, human uterus tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST58-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

130 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

COL1A1/Collagen Ⅰ

SYNONYMS

Alpha 1 type I collagen antibody; Alpha 2 type I collagen antibody; alpha 2 type I procollagen antibody; alpha 2(I) procollagen antibody; alpha 2(I)-collagen antibody; Alpha-1 type I collagen antibody; alpha1(I) procollagen antibody; CO1A1_HUMAN antibody; COL1A1 antibody; COL1A2 antibody; collagen alpha 1 chain type I antibody; Collagen alpha-1(I) chain antibody; collagen alpha-1(I) chain preproprotein antibody; Collagen I alpha 1 polypeptide antibody; Collagen I alpha 2 polypeptide antibody; collagen of skin, tendon and bone, alpha-1 chain antibody; collagen of skin, tendon and bone, alpha-2 chain antibody; Collagen type I alpha 1 antibody; Collagen type I alpha 2 antibody; EDSC antibody; OI1 antibody; OI2 antibody; OI3 antibody; OI4 antibody; pro-alpha-1 collagen type 1 antibody; type I proalpha 1 antibody; type I procollagen alpha 1 chain antibody; Type I procollagen antibody

SEQUENCE SIMILARITIES

Belongs to the fibrillar collagen family.

TISSUE SPECIFICITY

Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.

POST-TRANSLATIONAL MODIFICATION

Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.; Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline.; Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains.; O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.