COL1A1 Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH06-20] (HA751058)
Catalog# HA751058
Rabbit Monoclonal to COL1A1
Tested for
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WB
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IHC-P
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IF-Tissue
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IF-Cell
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FC
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IP
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IHC-Fr
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of COL1A1 on different lysates with Rabbit anti-COL1A1 antibody (HA751058) at 1/1,000 dilution.
Lane 1: HFF-1 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: Human lung tissue lysate (40 µg/Lane)
Lane 4: Mouse skin tissue lysate (40 µg/Lane)
Lane 5: Rat skin tissue lysate (40 µg/Lane)
Predicted band size: 139 kDa
Observed band size: 200/139 kDa
Exposure time: Lane 1-4: 1 minute; Lane 5: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751058) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HFF-1 cell lysate (20 µg/Lane)
Lane 2: NIH/3T3 cell lysate (20 µg/Lane)
Lane 3: Human lung tissue lysate (40 µg/Lane)
Lane 4: Mouse skin tissue lysate (40 µg/Lane)
Lane 5: Rat skin tissue lysate (40 µg/Lane)
Predicted band size: 139 kDa
Observed band size: 200/139 kDa
Exposure time: Lane 1-4: 1 minute; Lane 5: 3 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA751058) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.