CNTFR Rabbit Polyclonal Antibody (HA500319)
Catalog# HA500319
Rabbit Polyclonal to CNTFR
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
Lane 2: U-87 MG cell lysate (negative) (20 µg/Lane)
Lane 3: Neuro-2a cell lysate (20 µg/Lane)
Lane 4: Mouse brain tissue lysate (40 µg/Lane)
Lane 5: Mouse liver tissue lysate (negative) (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 60 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500319) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
CNTFR Rabbit Polyclonal Antibody (HA500319)
Immunogen
Synthetic peptide within human cntfr aa 1-50.
Host
Rabbit
Positive Control
SH-SY5Y cell lysate, Neuro-2a cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, SH-SY5Y, mouse heart tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:200
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 41 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CNTFR
SYNONYMS
Ciliary neurotrophic factor receptor alpha antibody;Ciliary neurotrophic factor receptor antibody;Ciliary neurotrophic factor receptor subunit alpha antibody;CNTF Receptor alpha antibody;CNTF receptor subunit alpha antibody;CNTFR Alpha antibody;CNTFR antibody;CNTFR-alpha antibody;CNTFR_HUMAN antibody
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
This gene encodes a member of the type 1 cytokine receptor family. The encoded protein is the ligand-specific component of a tripartite receptor for ciliary neurotrophic factor, which plays a critical role in neuronal cell survival, differentiation and gene expression. Binding of ciliary neurotrophic factor to the encoded protein recruits the transmembrane components of the receptor, gp130 and leukemia inhibitory factor receptor, facilitating signal transduction. Single nucleotide polymorphisms in this gene may be associated with variations in muscle strength, as well as early onset of eating disorders. Alternatively spliced transcript variants have been observed for this gene.
CITATIONS
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Xia Yu,et al
Artesunate-loaded solid lipid nanoparticles resist esophageal squamous cell carcinoma by inducing Ferroptosis through inhibiting the AKT/mTOR signaling
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WB
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Mouse
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Citation
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