PRODUCT CODE: ET7109-84

CLSTN1 Recombinant Rabbit Monoclonal Antibody [JE48-81] (ET7109-84)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of CLSTN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-84, 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse brain tissue lysate<br />
Lane 2: Siha cell lysate<br />
Lane 3: Mouse cerebellum tissue lysate<br />
Lane 4: A431 cell lysate
  • Western blot analysis of CLSTN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-84, 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse brain tissue lysate<br />
Lane 2: Siha cell lysate<br />
Lane 3: Mouse cerebellum tissue lysate<br />
Lane 4: A431 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CLSTN1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-84, 1/200)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CLSTN1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET7109-84, 1/200)  for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of CLSTN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7109-84, 1/2000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse brain tissue lysate
Lane 2: Siha cell lysate
Lane 3: Mouse cerebellum tissue lysate
Lane 4: A431 cell lysate

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CLSTN1 Recombinant Rabbit Monoclonal Antibody [JE48-81] (ET7109-84)

Immunogen

Synthetic peptide within c-terminal human clstn1.

Host

Rabbit

Positive Control

Mouse brain tissue lysate, Siha cell lysate, mouse cerebellum tissue lysate, A431 cell lysate, human liver cancer tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE48-81

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size: 110 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1000-1:5000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

CLSTN1

SYNONYMS

Alc alpha antibody; Alc-alpha antibody; Alcadein alpha 1 antibody; Alcadein alpha antibody; Alcadein-alpha antibody; alcalpha1 antibody; alcalpha2 antibody; Alzheimer related cadherin like protein antibody; Alzheimer-related cadherin-like protein antibody; C-terminal fragment 1-alpha antibody; Cadherin related family member 12 antibody; Calsyntenin 1 antibody; Calsyntenin1 antibody; CDHR12 antibody; CLSTN 1 antibody; Clstn1 antibody; CS1 antibody; CSTN1 antibody; CSTN1_HUMAN antibody; CTF1-alpha antibody; FLJ32258 antibody; KIAA0911 antibody; Non classical cadherin XB31alpha antibody; Non classical cadherin XB31alpha1 antibody; Non-classical cadherin XB31alpha antibody; PIK3CD antibody; SAlc-alpha antibody; XB31alpha antibody

TISSUE SPECIFICITY

Expressed in the brain and, a lower level, in the heart, skeletal muscle, kidney and placenta. Accumulates in dystrophic neurites around the amyloid core of Alzheimer disease senile plaques (at protein level).

POST-TRANSLATIONAL MODIFICATION

Proteolytically processed under normal cellular conditions. A primary zeta-cleavage generates a large extracellular (soluble) N-terminal domain (sAlc) and a short C-terminal transmembrane fragment (CTF1). A secondary cleavage catalyzed by presenilin gamma-secretase within the transmembrane domain releases the beta-Alc-alpha chain in the extracellular milieu and produces an intracellular fragment (AlcICD). This processing is strongly suppressed in the tripartite complex formed with APBA2 and APP, which seems to prevent the association with PSEN1.

SUBCELLULAR LOCATION

Endoplasmic reticulum. Golgi apparatus. Membrane. Nucleus.

FUNCTION

Induces KLC1 association with vesicles and functions as a cargo in axonal anterograde transport. Complex formation with APBA2 and APP, stabilizes APP metabolism and enhances APBA2-mediated suppression of beta-APP40 secretion, due to the retardation of intracellular APP maturation. In complex with APBA2 and C99, a C-terminal APP fragment, abolishes C99 interaction with PSEN1 and thus APP C99 cleavage by gamma-secretase, most probably through stabilization of the direct interaction between APBA2 and APP. The intracellular fragment AlcICD suppresses APBB1-dependent transactivation stimulated by APP C-terminal intracellular fragment (AICD), most probably by competing with AICD for APBB1-binding. May modulate calcium-mediated postsynaptic signals (By similarity).