CLIP170 Recombinant Rabbit Monoclonal Antibody [JE63-82] (HA721004)
Catalog# HA721004
Rabbit Monoclonal to CLIP170
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WB
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IF-Cell
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IHC-P
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Human
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF-7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 162 kDa
Observed band size: 162 kDa
Exposure time: 1 minute;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721004) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CLIP170 Recombinant Rabbit Monoclonal Antibody [JE63-82] (HA721004)
Immunogen
Synthetic peptide within human clip170 aa 1,389-1,438/1,438.
Host
Rabbit
Positive Control
Hela cell lysate, HepG2 cell lysate, MCF-7 cell lysate, HUVEC cell lysate, JAR cell lysate, human skin tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE63-82
RRID
APPLICATION DILUTION
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WB
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1:500
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IF-Cell
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1:50
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IHC-P
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 162 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CLIP170
SYNONYMS
CAP GLY domain containing linker protein 1 antibody;CAP-Gly domain-containing linker protein 1 antibody;CLIP 170 antibody;CLIP antibody;CLIP-170 antibody;CLIP1 antibody;CLIP1_HUMAN antibody;CYLN1 antibody;cytoplasmic linker 1 antibody;Cytoplasmic linker protein 1 antibody;Cytoplasmic linker protein 170 alpha 2 antibody;Cytoplasmic linker protein 170 alpha-2 antibody;Cytoplasmic linker protein CLIP 170 antibody;Reed Steinberg cell expressed intermediate filament associated antibody;Reed Sternberg intermediate filament associated protein 3 antibody;Reed-Sternberg intermediate filament-associated protein antibody;Restin antibody;RSN antibody
SUBCELLULAR LOCATION
Cytoskeleton, cytoplasm, cytoplasmic vesicle membrane, ruffle.
FUNCTION
Binds to the plus end of microtubules and regulates the dynamics of the microtubule cytoskeleton. Promotes microtubule growth and microtubule bundling. Links cytoplasmic vesicles to microtubules and thereby plays an important role in intracellular vesicle trafficking. Plays a role macropinocytosis and endosome trafficking. The protein encoded by this gene links endocytic vesicles to microtubules. This gene is highly expressed in Reed-Sternberg cells of Hodgkin disease. Several transcript variants encoding different isoforms have been found for this gene.
CITATIONS
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Yan Yuanliang,et al
Suppression of ITPKB degradation by Trim25 confers TMZ resistance in glioblastoma through ROS homeostasis
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WB
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Mouse
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Citation
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