Claudin 7 Mouse Monoclonal Antibody [6-G3] (M1412-2)
Catalog# M1412-2
Mouse Monoclonal to Claudin 7
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WB
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IF-Cell
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
Lane 1: SW480 cell lysate (20 µg/Lane)
Lane 2: MCF7 cell lysate (20 µg/Lane)
Lane 3: LNCaP cell lysate (20 µg/Lane)
Lane 4: Caco-2 cell lysate (20 µg/Lane)
Lane 5: A431 cell lysate (20 µg/Lane)
Lane 6: Mouse colon tissue lysate (40 µg/Lane)
Lane 7: Rat colon tissue lysate (40 µg/Lane)
Predicted band size: 22 kDa
Observed band size: 20 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1412-2) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Claudin 7 Mouse Monoclonal Antibody [6-G3] (M1412-2)
Immunogen
Synthetic peptide within human claudin 7 aa 162-211 / 211.
Host
Mouse
Positive Control
SW480 cell lysate, MCF7 cell lysate, LNCaP cell lysate, Caco-2 cell lysate, A431 cell lysate, mouse colon tissue lysate, rat colon tissue lysate, PC-12, human kidney tissue, mouse colon tissue, rat colon tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
6-G3
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:5,000
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 22 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
Claudin 7
SYNONYMS
CEPTR L2 antibody;CEPTRL 2 antibody;CEPTRL2 antibody;Claudin 1 antibody;Claudin 9 antibody;Claudin-7 antibody;Claudin1 antibody;Claudin7 antibody;Claudin9 antibody;CLD7_HUMAN antibody;CLDN 7 antibody;CLDN-7 antibody;CLDN7 antibody;Clostridium perfringens enterotoxin receptor like 2 antibody;CPETR L2 antibody;CPETRL 2 antibody;CPETRL2 antibody;Hs.84359 antibody
SEQUENCE SIMILARITIES
Belongs to the claudin family.
TISSUE SPECIFICITY
Expressed in kidney, lung and prostate. Isoform 1 seems to be predominant, except in some normal prostate samples, where isoform 2 is the major form. Down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC) (at protein level), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated.
SUBCELLULAR LOCATION
Cell membrane, Basolateral cell membrane, Cell junction, tight junction.
FUNCTION
Claudin-7 is a protein that in humans is encoded by the CLDN7 gene. It belongs to the group of claudins.Claudins, such as CLDN7, are involved in the formation of tight junctions between epithelial cells. Tight junctions restrict lateral diffusion of lipids and membrane proteins, and thereby physically define the border between the apical and basolateral compartments of epithelial cells. It is expressed in kidney, lung and prostate. Claudin-7 is down-regulated in breast cancers, including ductal carcinoma in situ (DCIS), lobular carcinoma in situ (LCIS) and invasive ductal carcinoma (IDC), as well as in several cancer cell lines. Loss of expression correlates with histological grade, occurring predominantly in high-grade lesions.
CITATIONS
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Rat tight junction proteins are disrupted after subchronic exposure to okadaic acid
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WB
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Rat
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Citation
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