Claudin 1 Recombinant Rabbit Monoclonal Antibody [PS01-43] (HA721248)
Catalog# HA721248
Rabbit Monoclonal to Claudin 1
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WB
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IHC-P
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IF-Tissue
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IF-Cell
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Human
Lysates/proteins at 10 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 19 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721248) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Claudin 1 Recombinant Rabbit Monoclonal Antibody [PS01-43] (HA721248)
Immunogen
Recombinant full length protein within human claudin 1.
Host
Rabbit
Positive Control
A431 cell lysates, A431, HepG2, human tonsil tissue, human skin tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PS01-43
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Tissue
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1:200
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Claudin 1
SYNONYMS
Claudin-1 antibody;Claudin1 antibody;CLD 1 antibody;CLD1 antibody;CLD1_HUMAN antibody;CLDN 1 antibody;Cldn1 antibody;ILVASC antibody;SEMP 1 antibody;SEMP1 antibody;Senescence associated epithelial membrane protein 1 antibody;Senescence associated epithelial membrane protein antibody;Senescence-associated epithelial membrane protein antibody
SUBCELLULAR LOCATION
Cell junction, Cell membrane, Membrane, Tight junction
FUNCTION
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions. Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell.
CITATIONS
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Sun Mengzhen,et al
Sodium butyrate administration improves intestinal development of suckling lambs
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WB
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Human
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Citation
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AMPK-PINK1/Parkin Mediated Mitophagy Is Necessary for Alleviating Oxidative Stress-Induced Intestinal Epithelial Barrier Damage and Mitochondrial Energy Metabolism Dysfunction in IPEC-J2
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WB
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Pig
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Citation
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Sauchinone alleviates dextran sulfate sodium-induced ulcerative colitis via NAD(P)H dehydrogenase [quinone] 1/NF-kB pathway and gut microbiota
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WB
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Mouse
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Citation
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Aixin Hu , Wenxia Huang , Xin Shu , Shiyue Ma , Caimei Yang , Ruiqiang Zhang , Xiao Xiao , Yanping Wu
Lactiplantibacillus plantarum Postbiotics Suppress Salmonella Infection via Modulating Bacterial Pathogenicity, Autophagy and Inflammasome in Mice
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WB
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Mouse
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Citation
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Root Extract of Lindera aggregata (Sims) Kosterm. Modulates the Th17/Treg Balance to Attenuate DSS-Induced Colitis in Mice by IL-6/STAT3 Signaling Pathway
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WB
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Mouse
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Citation
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