PRODUCT CODE: ET1704-50

Clathrin heavy chain Recombinant Rabbit Monoclonal Antibody [JA53-02] (ET1704-50)

  • Recombinant

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Clathrin heavy chain on Hela (1) and PC-12 (2) cell lysate using anti-Clathrin heavy chain antibody at 1/1,000 dilution.
  • Western blot analysis of Clathrin heavy chain on Hela (1) and PC-12 (2) cell lysate using anti-Clathrin heavy chain antibody at 1/1,000 dilution.
Western blot analysis of Clathrin heavy chain on Hela (1) and PC-12 (2) cell lysate using anti-Clathrin heavy chain antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Clathrin heavy chain Recombinant Rabbit Monoclonal Antibody [JA53-02] (ET1704-50)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, PC12, human adrenal gland tissue, human colon cancer tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA53-02

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

191/187 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Clathrin heavy chain

SYNONYMS

CHC17 antibody; Clathrin heavy chain antibody; Clathrin heavy chain 1 antibody; Clathrin heavy chain 2 antibody; Clathrin heavy chain like 1 antibody; Clathrin heavy chain on chromosome 17 antibody; Clathrin heavy polypeptide antibody; CLH 17 antibody; CLH 22 antibody; CLH-17 antibody; CLH1_HUMAN antibody; CLH17 antibody; CLH22 antibody; CLTC antibody; CLTCL antibody; CLTCL1 antibody; CLTCL2 antibody; CLTD antibody; Hc antibody; KIAA0034 antibody

SEQUENCE SIMILARITIES

Belongs to the clathrin heavy chain family.

TISSUE SPECIFICITY

Maximal levels in skeletal muscle. High levels in heart and testis. Low expression detected in all other tissues.

SUBCELLULAR LOCATION

Cytoplasmic vesicle membrane.

FUNCTION

Clathrin is a major cytosolic coat protein in pits and vesicles originating from the plasma membrane and the trans-Golgi network. In receptor-mediated endocytosis, receptor proteins are englufed by clathrin-coated vesicles. Clathrin is composed of three heavy chains and three light chains which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain binding site. The proximal segment of the Clathrin HC protein is essential for interactions between clathrin heavy chains and light chains which result in the formation of the triskelion structure.