PRODUCT CODE: ET1706-40

Citrate synthetase Recombinant Rabbit Monoclonal Antibody [JU80-30] (ET1706-40)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Citrate synthetase on Hela cell using anti-Citrate synthetase antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat kidney tissue<br />
       Lane 2: Hela<br />
 Lane 3: Mouse brain tissue
  • Western blot analysis of Citrate synthetase on Hela cell using anti-Citrate synthetase antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: Rat kidney tissue<br />
       Lane 2: Hela<br />
 Lane 3: Mouse brain tissue
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Citrate synthetase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of 293T cells with Citrate synthetase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Citrate synthetase on Hela cell using anti-Citrate synthetase antibody at 1/1,000 dilution.
Positive control:
Lane 1: Rat kidney tissue
Lane 2: Hela
Lane 3: Mouse brain tissue

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Citrate synthetase Recombinant Rabbit Monoclonal Antibody [JU80-30] (ET1706-40)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Mouse brain tissue lysate, rat kidney tissue lysate, Hela, rat epididymis tissue, human tonsil tissue, human kidney tissue, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU80-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Citrate synthetase

SYNONYMS

CISY_HUMAN antibody; Citrate synthase antibody; Citrate synthase, mitochondrial antibody; citrate synthetase antibody; Cs antibody; EC 2.3.3 antibody; EC 2.3.3.1 antibody

SEQUENCE SIMILARITIES

Belongs to the citrate synthase family.

POST-TRANSLATIONAL MODIFICATION

Methylated. Trimethylation at Lys-395 by CSKMT decreases citrate synthase activity.

SUBCELLULAR LOCATION

Mitochondrion matrix.

FUNCTION

Citrate synthase is found in nearly all cells capable of oxidative metabolism.The enzyme citrate synthase exists in nearly all living cells and stands as a pace-making enzyme in the first step of the citric acid cycle (or Krebs cycle). Citrate synthase is localized within eukaryotic cells in the mitochondrial matrix, but is encoded by nuclear DNA rather than mitochondrial. It is synthesized using cytoplasmic ribosomes, then transported into the mitochondrial matrix. Citrate synthase is commonly used as a quantitative enzyme marker for the presence of intact mitochondria.