PRODUCT CODE: ER40724

Chromogranin A Rabbit Polyclonal Antibody (ER40724)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of chromogranin A on PC12 cell lysates using anti-chromogranin A antibody at 1/2000 dilution.
  • Western blot analysis of chromogranin A on PC12 cell lysates using anti-chromogranin A antibody at 1/2000 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-chromogranin A antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Lovo cells with chromogranin A antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
Western blot analysis of chromogranin A on PC12 cell lysates using anti-chromogranin A antibody at 1/2000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Chromogranin A Rabbit Polyclonal Antibody (ER40724)

Immunogen

Peptide

Host

Rabbit

Positive Control

PC12, Lovo, mouse pancreas tissue, rat pancreas tissue, human colon cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

86 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000

  • IHC-P

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

Chromogranin A

SYNONYMS

CISY_HUMAN antibody; Citrate synthase antibody; Citrate synthase, mitochondrial antibody; citrate synthetase antibody; Cs antibody; EC 2.3.3 antibody; EC 2.3.3.1 antibody

SEQUENCE SIMILARITIES

Belongs to the chromogranin/secretogranin protein family.

POST-TRANSLATIONAL MODIFICATION

CgA is O-glycosylated.

SUBCELLULAR LOCATION

Cytoplasmic vesicle, secreted.

FUNCTION

Chromogranin A is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). Chromogranin A might promote the generation of secretory granules. It is used as an indicator for pancreas and prostate cancer. It might play a role in early neoplasic progression. It is also elevated in diabetes.