CHFR Rabbit Polyclonal Antibody (R1310-3)

UNIPROT #

Q96EP1

PROTEIN NAME

CHFR

SYNONYMS

Checkpoint with forkhead and ring finger domains antibody;Checkpoint with forkhead and RING finger domains protein antibody;Chfr antibody;CHFR_HUMAN antibody;E3 ubiquitin protein ligase CHFR antibody;E3 ubiquitin-protein ligase CHFR antibody;EC 6.3.2. antibody;FLJ10796 antibody;FLJ33629 antibody;RING finger protein 196 antibody;RNF 116 antibody;RNF 196 antibody;RNF116 antibody;RNF196 antibody;Ubiquitin ligase protein antibody

SEQUENCE SIMILARITIES

Belongs to the CHFR family.

TISSUE SPECIFICITY

Ubiquitous.

DEVELOPMENTAL STAGE

Weakly expressed in G1 phase, and highly expressed during S phase.

POST-TRANSLATIONAL MODIFICATION

Poly-ADP-ribosylated. In addition to binding non covalently poly(ADP-ribose) via its PBZ-type zinc finger, the protein is also covalently poly-ADP-ribosylated by PARP1.; Autoubiquitinated; may regulate its cellular level.; Phosphorylated by PKB. Phosphorylation may affect its E3 ligase activity.

SUBCELLULAR LOCATION

Nucleus

FUNCTION

E3 ubiquitin-protein ligase (CHFR) that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. CHFR is probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Marked reduction of CHFR expression was detected in primary tumors and decreased CHFR expression was linked to promoter hypermethylation. Restoration of CHFR expression by treatment with the microtubule stress agent nocodazole and the methyltransferase inhibitor 5-aza-2'-deoxycytidine has been reported.