PRODUCT CODE: ET7108-14

CHD1L Recombinant Rabbit Monoclonal Antibody [JG35-44] (ET7108-14)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

Western blot analysis of CHD1L on HepG2 cell using anti-CHD1L antibody at 1/1,000 dilution.
  • Western blot analysis of CHD1L on HepG2 cell using anti-CHD1L antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-CHD1L antibody. Counter stained with hematoxylin. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
  • Flow cytometric analysis of HepG2 cells with CHD1L antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of CHD1L on HepG2 cell using anti-CHD1L antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

CHD1L Recombinant Rabbit Monoclonal Antibody [JG35-44] (ET7108-14)

Immunogen

Recombinant protein within human chd1l aa 600-800.

Host

Rabbit

Positive Control

HepG2, human tonsil tissue, human colon cancer tissue, mouse testis tissue, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG35-44

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

101/90 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:100

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

CHD1L

SYNONYMS

ALC1 antibody; Amplified in liver cancer 1 antibody; Amplified in liver cancer protein 1 antibody; chd1l antibody; CHD1L_HUMAN antibody; CHDL antibody; Chromodomain helicase DNA binding protein 1 like antibody; Chromodomain-helicase-DNA-binding protein 1-like antibody; FLJ22530 antibody

SEQUENCE SIMILARITIES

Belongs to the SNF2/RAD54 helicase family.

TISSUE SPECIFICITY

Frequently overexpressed in hepatomacellular carcinomas.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

CHD1L (Chromodomain helicase DNA-binding protein 1-like) is a 897 amino acid protein encoded by the human gene CHD1L. The CHD family of proteins are ATP-dependent chromatin remodeling enzymes, which combine chromodomains, with SWI2/SNF2 ATPase/helicase motifs and DNA-binding capability. Chromodomains are protein regions of about 40-50 amino acid residues found in proteins associated with chromatin remodeling and manipulation. The domain is highly conserved among both plants and animals and is found in a large variety of proteins from many genomes. CHD1L and CHD1 share most homology within two internal helicase domains.