Cell Cycle Regulation Antibody Sampler Kit I (HAK21010)
Catalog# HAK21010
Reacts with
-
Human
Western blot analysis of Cdk2 on different lysates with Rabbit anti-Cdk2 antibody (ET1602-6) at 1/1,000 dilution.
Lane 1: Hela-si NT cell lysate
Lane 2: Hela-si Cdk2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minute;
ECL: merk
4-20% SDS-PAGE gel.
ET1602-6 was shown to specifically react with Cdk2 in Hela-si NT cells. Weakened band was observed when Hela-si Cdk2 sample was tested. Hela-si NT and Hela-si Cdk2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-6, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela-si NT cell lysate
Lane 2: Hela-si Cdk2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 1 minute;
ECL: merk
4-20% SDS-PAGE gel.
ET1602-6 was shown to specifically react with Cdk2 in Hela-si NT cells. Weakened band was observed when Hela-si Cdk2 sample was tested. Hela-si NT and Hela-si Cdk2 samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ET1602-6, 1/1,000) and Loading control antibody (Rabbit anti-GAPDH, ET1601-4, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-rabbit IgG-HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.