Cell Cycle Regulation Antibody Sampler Kit (HAK21039)
Catalog# HAK21039
Reacts with
-
Human
Western blot analysis of Cdk2 on different lysates with Rabbit anti-Cdk2 antibody (ET1602-6) at 1/500 dilution.
Lane 1: A549 cell lysate, 10 µg/Lane
Lane 2: F9 cell lysate, 10 µg/Lane
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-6) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: A549 cell lysate, 10 µg/Lane
Lane 2: F9 cell lysate, 10 µg/Lane
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1602-6) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.