CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02] (ET1605-4)
Catalog# ET1605-4
Rabbit Monoclonal to CDX2
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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FC
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CUT&Tag-seq
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Human
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Mouse
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Rat
Lane 1: Caco-2 cell lysate
Lane 2: LoVo cell lysate
Lane 3: AGS cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 34 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-4) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
CDX2 Recombinant Rabbit Monoclonal Antibody [SY09-02] (ET1605-4)
Immunogen
Synthetic peptide within human cdx2 aa 20-60.
Host
Rabbit
Positive Control
Caco-2 cell lysate, LoVo cell lysate, AGS cell lysate, LOVO, AGS, human appendix tissue, Human stomach cancer tissue, human colon cancer tissue, human colon tissue, mouse colon tissue, rat colon tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY09-02
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IF-Tissue
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1:200-1:2,000
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IHC-P
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1:500-1:20,000
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FC
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1:200-1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 34 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
CDX2
SYNONYMS
Caudal type homeo box 2 antibody;Caudal type homeo box transcription factor 2 antibody;Caudal type homeobox 2 antibody;Caudal type homeobox protein 2 antibody;Caudal type homeobox transcription factor 2 antibody;Caudal-type homeobox protein 2 antibody;CDX 2 antibody;CDX 3 antibody;CDX-3 antibody;Cdx2 antibody;CDX2_HUMAN antibody;CDX3 antibody;Homeobox protein CDX 2 antibody;Homeobox protein CDX-2 antibody
SEQUENCE SIMILARITIES
Belongs to the Caudal homeobox family.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation of Ser-60 mediates the transactivation capacity.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
In common with the two other Cdx genes, CDX2 regulates several essential processes in the development and function of the lower gastrointestinal tract (from the duodenum to the anus) in vertebrates. In vertebrate embryonic development, CDX2 becomes active in endodermal cells that are posterior to the developing stomach.These cells eventually form the intestinal epithelium. The activity of CDX2 at this stage is essential for the correct formation of the intestine and the anus.CDX2 is also required for the development of the placenta.
CITATIONS
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Microfluidic droplet encapsulation‐guided organoid growth promotes parental tumor phenotype recapitulation
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IHC
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Human
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Citation
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