Cdk8 Recombinant Rabbit Monoclonal Antibody [SN2030] (ET1611-99)
Catalog# ET1611-99
Rabbit Monoclonal to Cdk8
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WB
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Human
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Mouse
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Rat
Lane 1: A549 cell lysate
Lane 2: Human liver tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 58 kDa
Exposure time: seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-99) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Cdk8 Recombinant Rabbit Monoclonal Antibody [SN2030] (ET1611-99)
Immunogen
Synthetic peptide within human cdk8 aa 1-50 / 464.
Host
Rabbit
Positive Control
A549 cell lysate, Human liver tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN2030
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 53 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Cdk8
SYNONYMS
cdk8 antibody;CDK8 protein kinase antibody;CDK8_HUMAN antibody;Cell Division Kinase 8 antibody;Cell division protein kinase 8 antibody;Cyclin Dependent kinase 8 antibody;Cyclin-dependent kinase 8 antibody;K35 antibody;Mediator complex subunit cdk8 antibody;Mediator of RNA polymerase II transcription subunit cdk8 antibody;Protein kinase K35 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Cell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression. Another family of proteins, Cdk inhibitors, also plays a role in regulating cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1–3, PITALRE and PITSLRE. Large complexes containing Cdk8, cyclin C and the large subunit of RNA polymerase II have been identified. Cdk8 is thought to regulate RNA polymerase II function in conjunction with cyclin C. Cdk8 has been demonstrated to function as a transcriptional activator when fused to the DNA binding domain of GAL4.
CITATIONS
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Yan Yaoyao
The discovery of a novel pyrrolo [2, 3-b] pyridine as a selective CDK8 inhibitor offers a new approach against psoriasis
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WB
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Human
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Citation
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